Promotion Effect of Zn on 2D Bimetallic NiZn Metal Organic Framework Nanosheets for Tyrosinase Immobilization and Ultrasensitive Detection of Phenol

Abstract Environmental monitoring of pollutants is essential to guarantee the human health and maintain the ecosystem. The exploration of both simple and sensitive detection method has aroused widespread attentions. Herein, 2D bimetallic metal organic framework nanosheets (NiZn-MOF NSs) with tunable Ni/Zn ratio were synthesized, and for the first time employed to construct a tyrosinase biosensor. It is revealed that Zn element not only tuned the porosity structure and electronic structure of MOF NSs, but also modified the electrochemical activity. As a result, enzyme immobilization and electrochemical sensing performance of the NiZn-MOF NSs based biosensor were significantly enhanced by a suitable Zn addition. The fabricated tyrosinase biosensor exhibited excellent analytical detections, with a wide linear range from 0.08 μM to 58.2 μM, a high sensitivity of 159.3 mA·M-1, and an ultralow detection limit of 6.5 nM. In addition, the proposed biosensing approach also demonstrated good repeatability, superior selectivity, long-term stability, and high recovery for phenol detection in the real tap water samples.