Rapid Quantitative Detection of Chloramphenicol in Milk by Microfluidic Immunoassay

Abstract Chloramphenicol (CAP) is a toxic substance for human health, and detection of CAP residues in milk is necessary. However, most of the traditional CAP detection methods including high performance liquid chromatography-tandem mass spectrometry (HPLC-MS) and enzyme-linked immunosorbent assay (ELISA) are time-consuming and complicated. Herein, an automated microfluidics system for CAP detection in milk was developed. The residual CAP of multiple milk samples was quantitatively detected via competitive immunoassay in a single microfluidic chip simultaneously and automatically, and the reliability of the method was confirmed by flow cytometry. Completion of the detection by the system required less than 20 minutes and the cost for the detection of ten samples was about US$2.5. The limit of detection was 0.05 µg L−1, and the recovery rate of CAP in milk ranged from 91.3%-105.5%. The microfluidic system developed in this study exhibited considerable potential in the point-of-care testing (POCT) of CAP in milk.