Protective Effects of Vitamin E on Heart and Testis Histology Following MDMA (Ecstasy) Exposure in Mice

Background: Acute exposure to MDMA (methylenedioxymethamphetamine) has some adverse effects on reproductive and cardiovascular systems, possibly because of oxidative stress induction. Supplementary vitamins such as vitamin E can decrease the rate of oxidative stress and prevent the incidence of dysfunction in organs. Objectives: This study was designed to evaluate the adverse effects of MDMA exposure on the testis and heart tissue and elucidate the protective effect of vitamin E as a potent antioxidant. Patients and Methods: We assigned 28 male albino mice into four equal groups including control, MDMA, MDMA + vitamin E, and olive oil as a solvent of the vitamin. Mice were killed at the end of day 35 to conduct histological and plasma examinations. To find the relationship and make pair-wise comparisons, the one-way ANOVA test and Tukey test were used, respectively. Results: The mean values of seminiferous epithelial height (SEH), seminiferous tubular diameter (STD), spermatogenesis indices, and the average number of Leydig and Sertoli cells were significantly lower in testicular tissues of the MDMA group. The mentioned parameters were significantly higher in the MDMA + vitamin E group than in the MDMA group (P < 0.05). The apoptosis rate in both testis and heart tissues was significantly lower in the MDMA + vitamin E group than in the MDMA group (P < 0.05). Moreover, CPK and LDH as cardiac markers were significantly higher in the MDMA group than in the other groups. Conclusions: The results clearly suggest that vitamin E considerably attenuates the deleterious effects of MDMA.