HEp-2-adherent Escherichia coli strains associated with acute infantile diarrhea, São Paulo, Brazil.
2002
In this paired case-control study of infants with diarrhea in Sao Paulo, we examined the association between HEp-2–adherent Escherichia coli strains and diarrhea. We tested isolates from stool specimens of infants with diarrhea and matched controls in an HEp-2 cell adherence assay; we then hybridized isolates with DNA probes and identified enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), and diffusely adherent E. coli (DAEC). From 100 patient-control pairs, we isolated 78 HEp-2–adherent strains; of these, 61 strains were single pathogens identified in stools of infants with diarrhea. While typical EPEC was significantly associated with diarrhea (p 0.1). DAEC was isolated with equal frequency from patients and controls (p>0.1).
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