HPLC separation of phenolic phytochemicals from grape peels and seeds water extracts and their in-vitro antimalarial activities

Water extracts of three grape types black shami and white were separated using reversed phase HPLC PDA and their in vitroeffect on beta hematin inhibition were investigated The grape crude water extracts of peels and seeds impede the formation of beta hematin in vitroand therefore possess a significant antimalarial effect Black grape peel extract gave superior activity as revealed by its absorption value in comparison to CQ positive control In general black and shami peels water extracts were slightly more active than their corresponding seeds extracts while surprisingly the white grape peel extract was completely inactive The same inactivity was noticed when black shami and white grape juices as well as red and white wines were examined in vitro The lack in white grapes of the active phytochemicals that present at high levels in black and shami grapes explains the inactivity of the former Several secondary plant phenolic metabolites may be responsible for the antimalarial activity and subsequently one could infer that the antimalarial activity of water extract may be a result of the synergistic effect of its diverse phenolic phytochemicals