Analysis of Lomefloxacin by Spectrophotometry: Development of Simple Quantitative Analytical Method

An attempt has been made to develop a simple, sensitive and rapid spectrophotometric method of analysis for lomefloxacin in pharmaceutical dosage form using different media such as water, 0.1N NaOH, 0.1N HCl and Chloride (Cl) buffer as solvent system. These solvent systems were used to dissolve lomefloxacin and 0.105 mg/mL stock solutions were prepared for each solvent system. Lomefloxacin solution was scanned with UV- spectrophotometer and the absorption maximum (λmax) was found to be 287 nm. These solvent systems were successfully applied for analysis of five eye drop dosage form of lomefloxacin encoded as pp1, pp2, pp3, pp4 and pp5 marketed by five different pharmaceutical companies and the results were found to be satisfactory and reproducible. These solvent systems could be used for routine analysis of lomefloxacin in both research laboratories and pharmaceutical industries.