[Effect of hydroxyethyl starch 130/0.4 solutions with different volume on coagulant function in rats with severe sepsis].

Objective Aim to determine the effect of different dose of 6% 130/0.4 hydroxyethyl starch on coagulation function in rats model with severe sepsis by cecal ligation and puncture (CLP). Method 80 male SD rats were selected and randomly divided into the sham groups and the CLP groups, every group was divided into 4 small Groups again: before recovery (H0), 7.5 ml/kg group (H1), 15 ml/kg group (H2) and 30 ml/kg group (H3), n=10; The CLP groups were established by cecal ligation and perforation (CLP), the sham groups were the same as the CLP groups except for CLP, MAP was monitored for all rats during the experiment.After 4 hours by CLP, Procedure fluid resuscitation which received 6% hydroxyethyl starch 130/0.4 by respectively 7.5 ml/kg, 15 ml/kg, 30 ml/kg were started at a rate of 10 ml·kg-1·h-1, the sham groups were the same dose 6% hydroxyethyl starch 130/0.4 at the same time.All rats were taken for arterial blood harvest at 4 h after fluid infusion for arterial blood gases, blood routine examination, conventional coagulation function and thrombelastography (TEG). Results Compared with the sham groups, PO2in the CLP groups was not significantly different.pH, PCO2and MAP were significantly lower than the sham groups, Lac was significantly higher than the sham groups (P 0.05), R and K were significantly shorter than the sham groups, MA was significantly higher than the sham groups (P 0.05).Within CLP group, compared with H 0, R, K, α, MA and CI after fluid resuscitation were not significant different. Conclusion Low-dose (less than 30 ml/kg) 6% hydroxyethyl starch 130/0.4 in early fluid resuscitation have less effect on coagulation function in rats with severe sepsis. Key words: Hetastarch; Sepsis; BloodCoagulation; Thrombelastogrophy; FluidTherapy