Progesterone synthesis in the pure trophoblasts of human choriocarcinoma

Abstract A pure trophoblastic cell line of human choriocarcinoma has been successfully grown in tissue culture. Such a biological system provides an ideal model for the study of cellular metabolism in vitro . Cultures of this pure cell line were incubated with pregnenolone-7H 3 in the Waymouth's medium (MB 752-1), which contained no serum or exogenous enzyme. Based on the biochemical data and radiochemical purity criteria, the result demonstrated that the trophoblast of human choriocarcinoma synthesized progesterone in vitro .