[Tripterine inhibits all-trans retinoic acid-caused adhesion between leukemia cells and endothelial cells].

Objective: Increasing of adhesion between leukemia cells and endothelial cells during all-trans retinoic acid(ATRA) treatment plays an important role in retinoic acid syndrome.This work observed the effects of tripterine on this ATRA-caused increasing in adhesion.Methods: The effects of tripterine on ATRA-induced expressions of adhesive molecules in acute promyelocytic leukemia cell line NB4 and human umbilical vascular endothelial cells(HUVEC) were detected by flow cytometry.The effects of tripterine on adhesion between ATRA-treated NB4 and HUVEC were determined by adhesive assays.Results: ATRA caused remarkable elevation of intercellular adhesion molecule-1(ICAM-1) in NB4 cells,which could be significantly reduced by tripterine(P0.01).The expressions of E-selectin,vascular cell adhesion molecule-1(VCAM-1) and ICAM-1 in HUVEC were elevated by conditioned medium from ATRA-induced NB4(ATRA-NB4-CM)(P0.01),and inhibited by tripterine with inhibition rates being 25.3%,42.4% and 61.0% respectively.ATRA increased the adhesion between NB4 and HUVEC,which was reversed completely by tripterine.Conclusion: Tripterine can inhibit ATRA-caused adhesion between leukemia cells and endothelial cells,and it might be a potential agent for treating retinoic acid syndrome.