Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans.
2011
Streptococcus mutans dextranase hydrolyzes the internal α-1,6-linkages of dextran and belongs to glycoside hydrolase family 66. An N- and C-terminal deletion mutant of S. mutans dextranase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.6 A and belonged to space group P21, with unit-cell parameters a = 53.2, b = 89.7, c = 63.3 A, β = 102.3°. Assuming that the asymmetric unit of the crystal contained one molecule, the Matthews coefficient was calculated to be 4.07 A3 Da−1; assuming the presence of two molecules in the asymmetric unit it was calculated to be 2.03 A3 Da−1.
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