HPLC (high-performance liquid chromatography) detection method capable of simultaneously detecting contents of six amino acids in donkey-hide glue blood-supplementing preparation

The invention relates to a HPLC (high-performance liquid chromatography) detection method capable of simultaneously detecting contents of six amino acids in a donkey-hide glue blood-supplementing preparation. The method comprises the following steps: providing a donkey-hide glue blood-supplementing preparation sample; providing an amino acid standard sample; respectively adding the donkey-hide glue blood-supplementing preparation sample and the amino acid standard sample into phenylisothiocyanate-acetonitrile solution; uniformly mixing and standing, and respectively adding n-hexane; uniformlymixing and standing once again, respectively extracting lower-layer solutions, and filtering to obtain a derivatized donkey-hide glue blood-supplementing preparation sample solution and a derivatizedamino acid standard sample solution; sucking the derivatized donkey-hide glue blood-supplementing preparation sample solution and the derivatized amino acid standard sample solution, and injecting into a liquid chromatograph to perform amino acid detection. According to the invention, the contents of six major amino acids in the donkey-hide glue blood-supplementing preparation are detected by adopting reversed-phase high-performance liquid-phase, the sample treatment method is simple, the detection method has good specificity, repeatability and accuracy, and the internal quality of donkey-hide glue blood-supplementing granules can be well controlled.