Staphylococcus aureus. associated with beta-lactam resistance in Low-affinity penicillin-binding protein

METHODSBacteria. Anisogenicpairofstrains 27and27Rwaskindlysupplied by Richard P. Novick, New York City PublicHealthResearchInstitute. Methicillin resistance ofaclinicalisolate ofstrain 592wasintroduced into methicillin-sensitiverecipient strain 27 by transduction with phage 80ot. Themethicillin-resistant strain ColwassuppliedbyLeonSabath,