Cornea tissue repairing material and preparation method thereof

2015 
The invention discloses a cornea tissue repairing material and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a carboxymethyl chitosan solution and a gelatin solution respectively, mixing the carboxymethyl chitosan solution and the gelatin solution, adding a cross-linking agent into a mixed solution, stirring the mixed solution in a water bath kettle at 37 DEG C for 24h, and pouring a cross-linked solution into a mould; (2) pre-freezing the solution poured into the mould at minus 20 DEG C for 4h, pre-freezing the solution at minus 80 DEG C for 24h, and freeze-drying the solution in vacuum for 48h; (3) tabletting the product of freeze-drying to obtain a porous layer of the cornea tissue repairing material; and (4) coating one surface of the porous layer with a collagen solution containing a cross-linking agent, and drying a coating film naturally at room temperature to obtain the cornea tissue repairing material. The cornea tissue repairing material can be used for repairing and replacing injured cornea tissues in the medical field, and employs a simple preparation technology, is simple and easy in equipment and cheap and has easily available raw materials.
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