ADAPTIVE RESPONSE TO HIGH EXPOSURE OF CARCINOGENIC POLYCYCLIC AROMATIC HYDROCARBONS

Background and Aims: Ostrava Region in the Northern Moravia (Silesia) is the most polluted region in the Czech Republic by carcinogenic polycyclic aromatic hydrocarbons (c-PAHs) as benzo[a]pyrene (B[a]P). The aim of the study is to gain new knowledge on the mechanisms of the effects of complex mixtures bound on dust particles (c-PAHs) in the ambient air on biomarkers of exposure and effect during the period of inversion. Methods: In the Ostrava City were selected 78 volunteers- working in the office, as controls were city policemen from Prague (N=65). All volunteers were nonsmokers. The personal monitoring to c-PAHs was carried on in January-February 2010. The determined concentration of B[a]P was in the Ostrava region vs. Prague 14.8±13.3 vs. 2.80±1.87 ng/ m3. The impact of these concentration to biomarkers as DNA adducts, chromosomal aberrations, 8-oxodG, lipid peroxidation, and gene expression profiles are followed. DNA adducts were analysed in lymphocytes by 32P-postlabeling assay, chromosomal aberrat...