Effect of various antibiotics against the Actinobacillus lignieresi, Acinetobacter and Citrobacter species isolated from the frozen semen of cattle

Antibiogram  evaluation of Actinobacillus lignieresi, Acinetobacter and Citrobacter species were isolated from frozen semen of cattle. Different antibiotics such as amikacin, ofloxacin, neomycin, cephalexin, amoxicillin, ampicillin, gentamicin, sulphamethoxazole/trimethoprim, and erythromycin were tested against the isolated bacterial species. Amikacin, neomycin and gentamycin were highly effective against Acinetobacter. While the organism exhibited high resistance against the amoxicillin, erythromycin and cephalexin. Ofloxacin, amikacin, sulphamethoxazole and gentamycin were more effective against the Actinobacillus lignieresi than the neomycin and amoxicillin. Amikacin, neomycin, ofloxacin and gentamycin were highly effective against the Citrobacter species. Erythromycin, amoxicillin and cephalexin did not show response against the organism. Amikacin was found highly effective against the Acinetobacter, Actinobacillus lignieresi and Citrobacter species were isolated from frozen bovine semen.