Techniques, perspectives, and challenges of bioactive peptide generation: A comprehensive systematic review
2020
Due to the digestible refractory and absorbable structures of bioactive peptides (BPs),
they could induce notable biological impacts on the living organism. In this regard,
the current study was devoted to providing an overview regarding the available methods
for BPs generation by the aid of a systematic review conducted on the published
articles up to April 2019. In this context, the PubMed and Scopus databases were
screened to retrieve the related publications. According to the results, although the
characterization of BPs mainly has been performed using enzymatic and microbial
in-vitro methods, they cannot be considered as suitable techniques for further stimulation
of digestion in the gastrointestinal tract. Therefore, new approaches for both
in-vivo and in-silico methods for BPs identification should be developed to overcome
the obstacles that belonged to the current methods. The purpose of this review was to
compile the recent analytical methods applied for studying various aspects of foodderived
biopeptides, and emphasizing generation at in vitro, in vivo, and in silico.
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