Impact of the Technique of Mosquito Egg Impregnation on the Emergence Rate in the Insectarium

The breeding of the main vectors is essential for the conduct of many operational research programs. The right option of the technique of watering malaria vector larvae helps entomologists, insectarium managers and entomology technicians to produce high-quality Anopheles mosquitoes in the laboratory for different research and operational objectives. Evaporation of water in the tank causes suffering to the embryos stuck to the wall of the tank and due to lack of immersion, these embryos eventually die. We have experimented with a technique that consists in keeping the eggs in the center of the tank and preventing them from migrating towards the wall with a rectangular opening paper. The aim of this study is to understand the impact of the An. gambiae egg impregnation technique on the harvest rate in the insectarium. The hatching rate for all the eggs put in water in the paper trays is of the order of 83% to 99%. The results of paperless trays are necessarily worse than those of paper trays. In addition, the risk of mortality of larvae in the pupal stage is twenty (20) times lower with the paper tray than with the paperless tray. They therefore constitute basic indications to avoid a number of false steps from the outset.