Normal Mode Comparison Elucidates Dynein Walking Mechanism Triggered by ATP Hydrolysis

binding of the Ga subunit and therefore act as regulators of cell signaling. Ric8a is a cytoplasmic-receptor-independent-GEF that is known to interact with the C-terminus of Ga subunits, possibly in a similar manner as GPCR’s. In this study, domain and long-lived segmental motions involved in Ga subunit binding of the GEF Ric-8a and the subsequent nucleotide exchange was probed using single-molecule fluorescence spectroscopic methods.