The effect of bio-conditioning of titanium implants for enhancing osteogenic activity

Early and effective integration of titanium- based materials into bone tissue is of vital importance for long term stability of implants. Surface modification is commonly used to enhance cell-substrate interactions for improving cell adhesion, proliferation and activity. Here, the surface of titanium substrates and commercial implants were coated with blood (TiB), fetal bovine serum (TiF) and phosphate buffer saline (TiP) solution using spin coating process. Surface roughness and wettability of samples were measured using contact angel measurements and atomic force microscopy. The samples were then exposed to human osteoblast-like MG63 cells in order to evaluate adhesion, growth, differentiation and morphology on the surface of modified samples. Untreated titanium disks were used as control. The lowest roughness and wettability were found in unmodified titanium samples followed by TiP, TiF, and TiB. The percentage of cellular attachment and proliferation for each sample was measured using a MTT [3-4,5-dim...