Method for detecting copy number variation of human coronary heart disease susceptibility gene-lipoprotein a gene and kit thereof

The present invention relates to the gene copy number of real-time quantitative PCR, discloses a coronary heart disease-related human lipoprotein gene copy number in a detection method and kit, the method comprising the steps of: (1) extraction and purification of human peripheral blood genomic DNA; (2) preparation of plasmid standards positive; (3) establishing the Taqman quantitative PCR method. A lipoprotein gene as the target gene, the albumin gene as reference gene, and the establishment of quantitative PCR method for the desired gene using two primers double-probe method. Further establish the standard curve, and the feasibility of the standard curve parameters and by calculating the coefficient of variation of the replicate samples to assess the detection method. The methods and kits can play in the diagnosis of coronary heart disease early warning and early screening for high-risk groups an important role in helping to identify individuals at high risk of coronary heart disease, early implementation of prevention, monitoring, intervention and / or treatment, as far as possible to avoid the onset or delaying the onset .