Study on the Alkaloids in the Stems and Leaves of Stephania cepharantha(II)

Objective: To study the alkaloids in the stems and leaves of Stephania cepharantha Hayata.Methods: The dried stems and leaves of Stephania cepharantha Hayata were percolated with 95% ethanol and the solvent was removed by rotary evaporation to give a concentrate,and the concentrate was extracted by petroleum ether and chloroform.Column chromatograghy on MCI CHP 20P,silica gel,Rp-18,Sephadex LH-20 and polyamide were applied for the isolation and purification of the chloroform fraction.The structures were elucidated by their physicochemical properties and spectral data.Results: Five alkaloids were obtained and identified as,Stephasunoline(Ⅰ) Aknadinine(Ⅱ),Discretamine(Ⅲ),Acutumine(Ⅳ),Sinomenine(Ⅴ).Conclusion: Compounds Ⅰ、Ⅲ、Ⅳ are isolated from this plant for the first time,and compound Ⅳ is isolated from the genus for the first time.