Horse erythrocyte factor enhancing sulphonamide activity - I. Partial purification and data on the mode of action

The active principle of horse red blood cell haemolysate enhancing sulphonamide activityin vitro (HC factor) was partially purified by adsorption to DEAE-cellulose and subsequent fractionated elution. The properties of the factor, as disclosed so far, suggest that it may be a protein-containing substance of a rather high molecular weight. The HC factor counteracts the sulphonamide-antagonizing property of meat-extract, but not of peptone, of enzymic casein hydrolysate and of some known sulphonamide antagonists.