Assignments of 1HNMR fingerprint of the root bark of Celastrus angulatus

AIM To assign the 1HNMR finger print of the root bark of Celastrus angulatus. METHODS Silica gel column chromatography was used to separate the chemical constituents of CGE A of the root bark of C.angulatus. The characteristic signals of the 1HNMR finger print were assigned after determining the structures of the compounds isolated from CGE A. RESULTS 1HNMR fingerrints of the samples of C.angulatus collected from different regions showed highly characteristic features and reproducibility. Three compounds predominant in CGE A were isolated and their structures were determined by spectral analysis as: angulatin A (1), angulatin B (2) and angulatin C (3). CONCLUSION Compound 3 is a new compound. Compound 2 was isolated from C.angulatus for the first time. The 1HNMR finger print of CGE A of the root bark of C.angulatus showed mainly the characteristic signals of the above three compounds and might be utilized for the original authentication of this plant.