Determination of camptothecin in Camptotheca acuminata fruit from different habitats by TLCS

OBJECTIVE To establish a TLCS method for determination of camptothecin in Camptotheca acuminata fruit,and apply the method to investigate the quality of Camptotheca acuminata fruit from different producing areas.METHODS Silica gel H-CMCNa thin layer plate was adopted in the determination with chloroform-acetone(7:3) used as developer,Single-wavelength and linear scanning of TLC was used,and the detection wavelength was at 360 nm.RESULTS There was a good linear relationship for Camptothecin within the range of 0.0542-0.3252 μg.The regression equation was Y= 97.19X-55.07(r=0.9928).The average recovery was 97.13% with RSD of 1.76%(n=6).CONCLUSION The method is accurate and reliable,and the contents of camptothecin in Camptotheca acuminata fruit vary by different habitats.