Physical properties of virus causing cotton mosaic disease

The physical properties were found to be thermal inactivation point (TIP) 55–600C, dilution end point (DEP) 10-2 -10-3, dilutions and longevity in vitro (LIV) 5 hrs. In ELISA test it was found that the virus showing positive test only with anti serum of TSV of cowpea and cotton but negative reaction with PBNV of cowpea and cotton which clearly denied possibility of presence of PBNV in cotton producing these kinds of symptoms. ELISA report clearly showed that TSV antiserum of cowpea was showing positive results with clear chlorotic types of symptoms.