Culture,Identification of Mice Bone Marrow Mesenchymal Stem Cells and the Expression of Survivin

To culture and identify mice bone marrow mesenchymal stem cells (BMSCs),and to detect the expression of Survivin,BMSCs by whole bone marrow adherence method was adopted,charted the growth curves of the passage BMSCs,the specific surface markers were detected by flow cytometry (FCM),osteogenic and adipogenic induction was performed on BMSCs,expression of Survivin mRNA in BMSCs determined by RT-PCR.The results showed that the cells displayed fusiform shape as fibroblasts,the surface highly express CD29,CD34,CD44,SCA-1,lowly express CD117;the growth curve showed that cells grew slowly in the first three days,then growth accelerated and reached the top at the seventh day;after 20 days of osteogenic induction,alizarin red stain showed that alizarin red was positive in cells,and after 14 days adipogenic induction,oil red O stain showed that lipide sediment existed;Survivin mRNA was positive in BMSCs by RT-PCR.So BMSCs can be cultured abundantly by whole bone morrow adherence method,and expression of Survivin is positive,and indicating that Survivin may participate in the anti-apoptotic of BMSCs.