RNase P体外靶定切割人巨细胞病毒UL54 mRNA片段研究

Objective: To study the cleavage effect of M1 RNA, catalytic subunit of RNase P from Escherichia colion on UL54 mRNA encoding major DNA polymerase of human cytomegalovirus in vitro, and to explore the prospects of ribozyme as a novel antiviral agent. Methods: An external guiding sequence T6 specific to HCMV UL54 mRNA was designed and synthesized; specific cleavage activity of M1 RNA guided by T6 was observed in vitro. Results: With the guidance of EGS-T6, M1 RNA form Escherichia coli can block the expression of HCMV UL54 mRNA in vitro. Conclusions: EGS-T6 may fulfill the specific cleavage and be developed as a novel antiviral agent.