Comparison of results of the fluorescent method in the determination of atherogenic lipids and the data of biochemical methods

: Devices based on assessment of fluorescence of special "probes" incorporated in lipoprotein particles of the blood were developed at Research Institute of Physicochemical Medicine of the Ministry of Health of the Russian Federation. The resultant value is considered to reflect the sum of total cholesterol and triglycerides. The present study was aimed at elucidation of the relationship between the fluorescent and enzymatic methods, the enzymatic method realized by the Centrifikhem autoanalyzer. Coronary angiography during which blood for analysis by the two methods was collected was carried out in 182 patients aged 26 to 68 (mean age 49). Analysis of correlations revealed a direct relationship of the fluorescent value with the sum of total cholesterol and triglycerides, with triglycerides, with total cholesterol, and an inverse relationship with high-density lipoproteins. Using multifactorial regression analysis, the level of fluorescent parameter could be predicted only on the basis of triglyceride level and ratio of total cholesterol to high-density lipoproteins. Using the linear regression equation it is possible, if the fluorescent parameter value is available, to calculate triglyceride level. No relationship of the fluorescent parameter or triglycerides with the dissemination of coronary atherosclerosis, which is characteristic of total cholesterol sum, was detected.