Determination of total flavonoids in Lindera aggregata leaves

2010 
建立了AlCl3显色分光光度法测定乌药叶中总黄酮含量的方法.研究表明,在弱酸性条件下,乌药叶提取液中总黄酮与AlCl3形成稳定的黄色配合物,在波长415 nm处测定显色液的吸光度确定总黄酮含量.以芦丁为标准品,样品总黄酮在0.002~0.020 mg/mL范围内,线性关系良好,其回归方程为A=30.076C+0.006 5,R2=0.999 6,平均加样回收率=101.0%,RSD=1.27%(n...
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