Morphologic evaluation of dental pulp vascularity during DMBA experimental administration

7,12 - Dimethylbenz(a)anthracene (DMBA) is a well known carcinogen and immunosuppressor. Oral administrated DMBA circulates in the bloodstream and rich vascularized tissues like dental pulp can be particularity sensible to this compound effect. In this study we evaluate dental pulp morphologic changes during DMBA per os experimental administration. Control and test groups were constituted by ten male Wistar rats each, 8 weeks old. Control group was maintained without manipulation and test group was submitted to daily administration of 20mg/kg DMBA dissolved in olive oil and administrated by oral gavage, both during 4 weeks. All animals were sacrificed at the end of 4 weeks period and a complete necropsy was performed. Slides of dental pulp were obtained from the lower incisors dental root medium third and hematoxylin-eosin coloration was performed. Slides were observed in light microscopy and morphometric analysis was performed, evaluating areas of dental pulp vascularity, calcification and presence of in...