Preventive effects of cyclosporine a combined with prednisolone and melatonin on contralateral testicular damage after ipsilateral torsion-detorsion in pubertal and adult rats.

Purpose: We compared the preventive effects of cyclosporine A combined with prednisolone and melatonin (Sigma-Aldrich®) on damage to the contralateral testis after ipsilateral testicular torsion-detorsion between pubertal and adult rats.Materials and Methods: We divided pubertal and adult Sprague-Dawley® rats into groups 1—sham operation, 2—detorsion, 3—detorsion plus cyclosporine A with prednisolone and 4—detorsion plus melatonin. After 4 hours of ipsilateral testicular torsion we treated the rats with detorsion only or with detorsion plus drug depending on the group.Results: Seminiferous tubule diameter and germ cell layer thickness were greater in pubertal group 3 and adult group 4 than at each age in group 2 (each p <0.05). The number of spermatids per tubule was greater in pubertal groups 3 and 4, and in adult group 4 than at each age in group 2 (each p <0.05). Of pubertal rats those in groups 3 and 4 had fewer TUNEL positive cells than group 2 (p = 0.061 and 0.057, respectively). Of adult rats the n...