Effect of Kegu recipe on angiogenesis stimulating factors in the steroid-induced avascular necrosis of the rabbit femoral head

Objective To investigate the effect of Kegu recipe on angiogenesis stimulating factors (VEGF mRNA)in the steroid -induced avascular ecrosis of the rabbit femoral head. Methods A total of 36 adult New Zealand rabbits were randomly divided into three groups equally: the control group, the model group, and the Kegu recipe group. The experimental animals were partial executed at fourth, eighth, twelfth weeks so that to access the main blood vessels of the femoral head including the medial femoral circumfles artery, femoral medial femoral circumflex artery and femoral vein. The specimen used the in situ hybridization method to measure VEGF levels and underwent routine histologic examination. Results At fourth week, there was no statistical significance between three groups though all groups showed positive staining in VEGF mRNA of endarterium and vein intima(F=1.36,2.46,P0.05). At eighth and twelfth week, the differences of VEGF mRNA positive staining in endarterium and vein intima were statistical significance (F=14.58,11.35,P0.05). At eighth week, the VEGF mRNA positive staining in endarterium and vein intima of model group was significantly lower than Kegu recipe group and control group(t=12.13,15.23,19.36,18.25,P0.05). At twelfth week, the differences of the VEGF mRNA positive staining in endarterium and vein intima were statistical significance between model group and control group as well as between Kegu recipe group and model group(t=12.09,19.35,20.37,16.74,P0.05). The differences of the VEGF mRNA positive staining in vein intima between Kegu recipe group and control group had no statistical significances (t =2.59,P 0.05). Conclusions Kegu recipe could improve the presence of VEGF mRNA, which may boost the biological activity of the major factors for angiogenesis in the vascular wall of the steroid-induced necrosis of the femoral head.