MMP8 polymorphism is associated with susceptibility to osteonecrosis of the femoral head in a Chinese Han population

// Feimeng An 1, 2, * , Jieli Du 2, * , Yuju Cao 3 , Jianping Shi 4 , Yongchang Guo 3 , Tianbo Jin 5 , Jian Li 3 , Junyu Chen 1, 2 , Ping Li 2 , Mei Dong 2 , Guoqiang Wang 2 , Jianzhong Wang 2 1 Inner Mongolia Medical University, Hohhot, Inner Mongolia, China 2 Department of Orthopedics and Traumatology, The Second Affiliated Hospital of Inner Mongolia University, Hohhot, Inner Mongolia, China 3 Zhengzhou TCM Traumatology Hospital, Zhengzhou, Henan, China 4 Department of TCM Diagnosis, Inner Mongolia Medical University, Hohhot, China 5 MOE Key Laboratory of Resource Biology and Modern Biotechnology, Northwest University, Xi’an, China * These authors contributed equally to this work and should be considered co-first authors Correspondence to: Guoqiang Wang, email: wangguoqianggq@163.com Jianzhong Wang, email: wangjianzhong0503@126.com Keywords: MMP8, single nucleotide polymorphisms, osteonecrosis of the femoral head, association study Received: October 07, 2016     Accepted: January 09, 2017     Published: February 16, 2017 ABSTRACT Osteonecrosis of the femoral head (ONFH) is an orthopedic refractory disease that adversely affects quality of life. Matrix metalloproteinase-8 (MMP-8) produced by the bone marrow has been implicated in the degradation of collagen during bone development. We assessed whether MMP8 polymorphisms are associated with ONFH. In a case-control study, using χ 2 tests and genetic model analyses, we genotyped 5 MMP8 single-nucleotide polymorphisms (SNPs) in 585 ONFH patients and 507 healthy control subjects in a Chinese Han population. The MMP8 rs11225394 SNP was associated with an increased risk of ONFH in an allele model (OR=1.34; 95% CI, 1.003-1.786, P=0.047). In addition, rs11225394 was associated with an increased risk of ONFH in a dominant model (OR =1.39, 95% CI, 1.02–1.89, P=0.036), over-dominant model (OR=1.39, 95% CI, 1.02–1.89, P=0.038), and log-additive model (OR =1.36, 95% CI, 1.01–1.84, P=0.039). After adjusting for age and gender, rs11225394 was associated with ONFH in a dominant (OR =1.44, 95% CI, 1.05–1.96, P=0.023), over-dominant (OR =1.44, 95% CI, 1.05–1.98, P=0.022), and log-additive model (OR =1.40, 95% CI, 1.04–1.90, P=0.027). These results provide the first evidence that MMP8 SNP at the rs11225394 locus is associated with the increased risk of ONFH in Chinese Han population.