ОПРЕДЕЛЕНИЕ ОСТАТОЧНЫХ ОРГАНИЧЕСКИХ РАСТВОРИТЕЛЕЙ МЕТОДОМ ГАЗОВОЙ ХРОМАТОГРАФИИ В СУБСТАНЦИИ (2R,4R,4аR,7R,8aR)-4,7-ДИМЕТИЛ-2-(ТИОФЕН-2-ИЛ)ОКТАГИДРО-2H-ХРОМЕН-4-ОЛ, ОБЛАДАЮЩЕЙ АНАЛЬГЕТИЧЕСКОЙ АКТИВНОСТЬЮ
2018
Introduction of a substance into medical practice requires the indispensable evidence of the substance purity as early as the stage of preclinical testing. The content of residual organic solvents, which can affect the pharmacological activity of the drug, is one of the indicators of the substance quality control. Four solvents are used during synthesis and isolation of the substance: dichloromethane, ethyl acetate, methyl tert -butyl ether (MTBE), and n -hexane. Two methods of determining residual organic solvents in (2 R ,4 R ,4 aR ,7 R ,8 aR )-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2 H -chromen-4-ol using gas chromatography are offered: i) introduction of a gas sample and separation of the components on a commercial HP-5ms capillary column (30 m × 0.35 mm × 0.25 im); ii) introduction of a liquid sample and separation on a capillary column (30 m × 0.32 mm × 0.30 im) filled with a modified poly(1-trimethylsilyl-1- propyne) (PTMS). Since the first method provides only determination of n -hexane and ethyl acetate, the procedure was developed and validated for determination of four residual solvents, i.e., dichloromethane, ethyl acetate, methyl n -butyl ether, and n -hexane in test substance using gas chromatography on the column with a modified PTMSP-based phase. We detected trace amounts of ethyl acetate and methyl n -butyl ether; the content of dichloromethane and n -hexane in the substance appeared below the detection limits. The average content of ethyl acetate and MTBE in the substance for 9 parallel determinations (0.10 and 0.20 ppm) appeared 5 and 2.5 × 104 times lower than maximum permissible content (5000 ppm), respectively. The developed technique was assessed for: specificity, linearity of the calibration curve, correctness, precision at the level of the repeatability and intralaboratory precision, and stability of the chromatographic system. Standard deviations in the determination of ethyl acetate and MTBE in the substance do not exceed 0.03 and 0.09, respectively. The procedure is specific, accurate and reliable for determination of the residual organic solvents in the substance.
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