Amperometric Biosensors Based on Choline Oxidase Entrapped in Polyacrylamide Microgels

2007 
A choline amperometric biosensor has been designed using as biological component choline oxidase (ChOx) entrapped in polyacrylamide microgels. The working electrode was prepared by holding the enzyme loaded microgels on a platinum electrode by a dialysis membrane. It was found that the optimum microgel cross-linking required to retain ChOx and to allow the diffusion of choline was 7.0%. The response of the biosensor was optimized in relation to pH, temperature and working potential and the following optimal values were obtained: pH 9.0, temperature range between 20 and 308C, and potential þ 0.6 V. Under optimal conditions the sensitivity for choline was 17.45 mA M � 1
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