Cross-country statin biosynthetic gene cluster and its application

The present invention relates to off-road statin biosynthetic gene cluster, in particular, is produced from a small single sp.TP?A0468 of Micromonospora sp anthracycline antibiotic having antitumor activity - Cloning of cross-country statin biosynthetic gene cluster , sequencing, analysis, function and its application. Entire gene cluster contains 55 genes: 17 type II polyketide synthase (PKS) associated gene; 8 nonribosomal polyethylene synthetase (the NRPS) related gene; 9 glycosyl biosynthesis-related gene; six specific after the modified gene; resistance gene 7; 5 and 3 regulate gene no clear functional genes. The synthetic gene by genetic manipulation of the biometric statins block the biosynthesis off-road, so that the yield or change, or produce new compounds. Genetic engineering can be used in the gene cluster of anthracycline compound, protein expression, enzyme-catalyzed reactions and the like, it can also be used to seek and find a pharmaceutical, agricultural or industrial compound or a gene, protein.