Breeding the Lines with Resistance to Fusarium Head Blight of Wheat Cultivar Yangmai 13 by Molecular Marker Assisted Selection

2010 
Molecular marker-assisted method combined with backcrossing was used to improve the disease resistance of Yangmai 13 to Fusarium head blight(FHB) and increase its production and profits. Using the resistant cultivar Sumai 3 as the donor parent of FHB resistance gene Fhb1 and Fhb2,and Yangmai 13 serve as the receptor and the recurrent parent,which was planted largest in the middle and lower reaches of the Yangtze River with weak gluten character and poor resistance to FHB,we successfully characterized eight lines with agronomic traits resemble to Yangmai 13 by using SSR markers liked to resistance genes.Finally,three lines with genetic background consistence with the recurrent parent were identified by genetic molecular markers,which were used for further tests of pathogen inoculation and main quality traits.The three lines developed through this method,were named as R Yangmai 13-2,R Yangmai 13-7 and R Yangmai 13-8,carrying Fhb1,Fhb2,good agronomic traits and soft quality.The disease incidence of them decreased by 78.82%~84.58%and wheat yield improved by 17.24%~26.72%,respectively compared to Yangmai 13,and could replace the susceptible FHB Yangmai 13 for large plantation.This study demonstrates that molecular marker-assisted selection with molecular markers tightly link to the major resistance genes offers opportunities for transferring FHB resistance in wheat breeding.
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