Primary Antibody Selection and Blocking Techniques for Immunohistochemistry.
2022
Immunohistochemical procedures are complex, multiparameter assays that must take into account many factors during protocol development. The overall goal of a successful immunohistochemistry protocol is to produce a specific, sensitive, robust, and reproducible stain. Each step in the protocol has an important role in this outcome. This chapter focuses on primary antibody selection and techniques for blocking various undesired staining reactions.
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