Progress on the cryodamage of frozen-thawed boar spermatozoa.

The morphological changes of acrosome,plasma membrane and mitochondrion of frozen-thawed boar spermatozoa and their effect on the fertilization ability of sperm were reviewed,and the changes of biomacromolecules of frozen-thawed boar spermatozoa such as,protein,DNA,etc,and their influence on the quality and fertility of frozen-thawed boar spermatozoa were also analysed.As a result,the conclusion was drawn that the effect of quality and quantity of biomacromolecules such as protein,DNA,are the cryodamage essence of frozen-thawed spermatozoa in the process of cryopreservation.The application of the advanced proteomics on study of the cryodamage mechanism of frozen-thawed boar spermatozoa will contribute to the elucidation of the molecular mechanism of freezing injury,and will provide rationale support for the technique breakthrough of the cryopreservation of boar semen.