A novel biomarker for beryllium sensitization in humans. 1997 annual progress report

'Overall, this project is designed to identify the beryllium reactive T-cell clones that are proliferating in vivo in individuals sensitized to beryllium. The basic method for identifying such clones is the hprt T-cell mutation assay. The rationale is that in vivo proliferating T-lymphocytes are more likely to undergo hprt mutations and/or be included in hprt mutant fractions of T-cells isolated from peripheral blood. T-lymphocytes isolated as hprt mutants from beryllium sensitized individuals are propagated in vitro and characterized for T-cell receptor (TCR)/3 gene usage patterns and antigen reactivity. Results will be compared with similar characteristics determined for T-cell clones from the same individuals that were developed in vitro from peripheral blood lymphocytes by beryllium stimulation. This research project has several specific aims. Progress for the year 10/1/96 to 9/30/97 is given.'