Method for inducing embryonic stem cell into pancreatic tissue-like cells

2013 
The invention discloses a method for inducing an embryonic stem cell into pancreatic tissue-like cells. The method comprises the following steps of: using a hanging-drop preparation method to differentiate an embryonic stem cell into three germ layer cells and using a immunomagnetic bead cell sorting method to purify finalized inner germ layer cells; further inducing the finalized inner germ layer cells so as to differentiate the cells into pancreatic precursor cells; and further differentiating the pancreatic precursor cells outside the body so as to form pancreatic tissue-like cells with high amylase secretion capability. The technical scheme of the invention adopts a plurality of induction factors for induced differentiation in different stages, which proves that the embryonic stem cell has the potentiality of being differentiated into pancreatic exocrine cells, and an effective way for inducting the embryonic stem cell into pancreatic tissue-like cells is found out, so that the pancreatic precursor cells are possible to recover injury of pancreas, and more cell sources are provided for recovering pancreas.
    • Correction
    • Source
    • Cite
    • Save
    • Machine Reading By IdeaReader
    0
    References
    0
    Citations
    NaN
    KQI
    []