Molecular characterization and phylogenetic analysis of unusual x-type HMW glutenin subunits from 1sl genome of Aegilops longissima
2015
Wheat related diploid species Ae. longissima (2n=2x=14, SlSl) has extensive
storage protein variations that may provide useful gene resources for wheat
quality improvement. In this work, five novel 1Sl-encoded x-type high
molecular glutenin subunits (HMW-GS) were identified and designated as
1Slx-123, 1Slx-129, 1Slx1-136, 1Slx2-136 and 1Slx2.2, respectively. Their
complete open reading frames (ORFs) were cloned and sequenced by AS-PCR,
which contained 2874 bp (956 aa) for 1Slx-123, 2946 bp (979 aa) for 1Slx-129,
2901 bp (965 aa) for 1Slx1-136, 2982bp (991 aa) for 1Slx2-136 and 2928 bp
(974 aa) for 1Slx2.2. Molecular characterization demonstrated that five
unusual subunits had greater repetitive domains resulted from a larger
fragment insertion (74-113 aa). Particularly, 1Slx-129 had an extra cysteine
residue at the position 109 due to a TAT → TGT dot mutation, which may
improve the formation of superior gluten macropolymer. Our results suggest
that these unusual HMW-GS could be served as potential superior gene
resources for improving wheat gluten quality. Phylogenetic analysis revealed
that HMW-GS genes from Glu-1Sx genomes had close evolutionary relationships
with those of Glu-1Dx genome while sequences from Ae. speltoides aligned with
those of B genome. [Projekat Ministarstva nauke Republike Srbije, br.
TR-31069]
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