Method for inhibiting non-specific adsorption of solid material surface

The invention relates to a method for inhibiting non-specific adsorption of a solid material surface, in particular to a method for solving the problem of non-specific adsorption of large biological molecule, germ, cell and the like through combining the non-ionic surface active agent on fluoride monomolecular layer surface or nylation monomolecular layer surface. The invention comprises: a layer of fluoride monomolecular layer film or nylation monomolecular layer film is modified on the solid material surface, and when using mixed non-ionic surface active agent to prepare buffer solution containing large biological molecule, the large biological molecule can inhibit non-specific adsorption of large biological molecule in the buffer solution together with the mixed non-ionic surface active agent and the fluoride monomolecular layer film or nylation monomolecular layer film. Through the cooperation of non-ionic surface active agent, and fluoride surface or nylation surface, the invention can simply, effectively and fast prepare the large biological molecule microarray detection part, is easy to operate, has low cost and is applicable for mass production.