Detection of serum HBV-PreS1 and HBV-DNA in HBeAg-negative hepatitis B patients with

OBJECTIVE To analyze the clinical significance of serum HBV-PreS1 and HBV-DNA in patients with Hepatitis B whose HBeAg were negative. METHODS Serum specimens of 114 Hepatitis B patients with HBeAg negative were collected.HBV-DNA was detected using the real-time fluorescent quantitative PCR method and HBV-PreS1 was measured by ELISA. RESULTS Of all the 114 serum specimens,69 cases of HBV-DNA and 77 cases of HBV-PreS1 was positive.The detection ratio was 60.5%(69/114) and 67.5%(77/114),respectively.It showed that there were good relationship between HBV-PreS1 and HBV-DNA and there were no significant difference between them in patients with Hepatitis B whose HBeAg were negative(χ2=1.45,P0.05).When the HBV-DNA copies was between 103 and 107,the detection ratio of HBV-PreSl gradually increased with HBV-DNA. CONCLUSION Detection of HBV-PreS1 can serve as an effective alternative for the measurement of HBV-DNA in HBeAg-negative Hepatitis B patients.