Complement-bound human antibodies to vaccinia virus B5 antigen protect mice from virus challenge

Evaluation of: Benhnia MR, McCausland MM, Laudenslager J et al. Heavily isotype dependent protective activities of human antibodies against the vaccinia virus extracellular virion antigen B5. J. Virol. 83, 12355–12367 (2009).The mechanism of protection afforded by vaccinia virus (VACV) – the smallpox vaccine – is a key issue for the development of modern vaccines and countermeasures. Antibodies to VACV antigens of the extracellular virion (EV) form play a central role in protection against poxvirus diseases in animal models, and contribute to the protection of immunized humans against poxviruses. B5, a viral EV protein, is conserved among different orthopoxviruses and antibodies to B5 that protect mice against VACV challenge. Antibodies to B5 are primarily responsible for neutralization of vaccinia EVs, yet the mechanism of EV neutralization by antibodies to B5 is not fully understood. The paper under evaluation demonstrates that most of the neutralization in vitro and protection in vivo in a mouse model,...