Evaluation of the Xpert HIV-1 viral load assay on a wide panel of HIV-1 variants

s / Journal of Clinical Virology 70 (2015) S1–S126 S83 sis of log10 concentrations of positive (n=22) clinical specimens showed correlation between the two methods (slope, 2.1 [CI95%: 0.54–3.59] and y-intercept value, −3.5 [CI95%: −7.82 to 0.88]). The Bland–Altman representation showed that viral loads obtained with the Iam CMV Q-LAMP Quantitative assay were lower than those obtained with the reference technique: the average deviations was 0.21 log10 IU/ml (standard deviation=1.32). The mean viral load obtained with the Iam CMV Q-LAMP Quantitative assay was 2.85 log10 IU/ml; it was 3.06 log10 IU/ml with the reference technique. 16 samples presented with a deviation above 0.5 log10 IU/ml. Conclusion: This study showed that good analytical and clinical performances of the Iam CMV Q-LAMP Quantitative assay make possible the use of this technique in the monitoring of CMV-DNA in plasma. http://dx.doi.org/10.1016/j.jcv.2015.07.193