Study of metabonomics on pharmacological action appraisal Rhizoma Coptidis in rats

Objective: HPLC-MS/MS-based metabonomics method was used to find the possible biomarker of Rhizoma Coptidis in rat urine.Method: Sprague-Dawley rats were successively administrated 7 g·kg-1 aqueous extract of Rhizoma Coptidis for 30 days,urine were collected by metabolism cages and detected by using the HPLC-MS-MS.All dates were analyzed by the principal component analysis(PCA) through using the SIMCA-P 10.0 software.Result: The PCA demonstrated that the metabolome between treated group and control group had difference in rat urine sample after of 22 days administrated,for treated group 169 kinds of biomarkers were found including oxalacetic acid,malic acid,2-ketoglutaric acid,NE,arachidonic acid,5-HIAA and other compounds,the result was consistent with pharmacological effects of R.coptidis,such as antiinflammatory,inhibiting biosynthesis of CA biosynthesis,anti-central nerve and energy metabolism inhibition.Conclusion: Metabonomics may be available in pharmacological action evaluation of drugs.