Most frequent mutational events of home box 13 gene in prostatic adenocarcinoma and correlation with tumor characteristics

Abstract The home box transcription factor gene (HOXB13) plays a certain role in human development and prostate tumorgenesis. Genetic alterations of HOXB13 help to explore the suspected role in prostate cancer. To determine the prevalence and clinical correlates of high-frequent mutational events in home box transcription factor (HOXB13); DNA samples from 50 men treated at the military hospital in Rabat, Morocco, were genotyped and confirmed by Sanger sequencing. The frequency and distribution of high frequent mutations were determined according to patient pathological characteristics. Within coding region of HOXB13; the Frameshift mutation: c.575delG (p.Gly192Valfs) with frequency of 5/50 (10%), and nonsense mutation: c.261C > A (p.Tyr87Ter), frequency: 6/50 (12%) and Missense mutations: c.665C > G (p.Pro222Arg), c.643C > T (p.Arg215Cys), c.776A > C (p.Gln259Pro), c.757A > C (p.Thr253Pro) with frequency rates: 10/50 (20%), 9/50 (18%), 8/50 (16%), and 7/50 (14%) respectively, were the most frequent mutations. Patients carrying one or more high frequent mutation at the same time treated with radical prostatectomy were: 9/35(26%), and 8/35 (23%) of them have pathological Gleason score >7 [p values (carriers vs. non carriers) were: 0.199266 and 0.75873 respectively]. The previously described germline mutation G84E was not found in this population. No significant differences between common mutations carriers and noncarriers, regarding selected tumor characteristics of prostate cancer. The clinical significance of Novel mutations detected on prostate cancer development still unknown. Our findings provide insight into the most common mutations of HOXB13 in prostate cancer and highlight prospective opportunities to analyze its clinical significance.