Research Article Modulation of inflammatory mediators by coumarinolignoids from Cleome viscosa in female swiss albino mice

The effect of coumarinolignoid cleomiscosins A, B and C isolated from the plant Cleome viscosa on inflammatory mediators were studied in female swiss albino mice. A mix- ture of coumarinolignoid A, B, and C at 10, 30 and 100 mg/kg body weight once a day for 14 consecutive days were admin- istered orally to the mice. Pro-inflammatory mediators such as IL-6, TNF-a and Nitric oxide were estimated from culture supernatant obtained from peritoneal macrophages stimulated by LPS and anti-inflammatory mediator IL-4 was estimated from culture supernatant obtained from spleenocytes stimu- lated by Con-A. For further confirmation, expressions of in - flammatory mediators from serum and mortality rate were studied in LPS-induced toxicity model in mice. Pro-inflam - matory mediators expression were significantly (P <0.05) decreased in coumarinolignoids treatment group in dose de- pendent manner, whereas the anti-inflammatory mediator ex - pression was significantly increased in coumarinolignoids at 10 mg/kg treatment. Mortality rate was also significantly re - duced in treatment group in LPS-induced toxicity model. The result of this study concluded that the oral administration of coumarinolignoids inhibited the pro-inflammatory mediators and enhances the production of anti-inflammatory mediator in dose dependent manner.