소청룡탕이 LPS로 유도된 폐손상 동물모델에 미치는 영향

Objectives : In present study, we investigated a therapeutic effect and optimum dose of Socheongryong-Tang (SCT) on LPS-induced lung inflammation rats model. Methods : Male Sprague-Dawley rats (260 ± 10 g) were divided into 12 groups : Group 1 included the normal rats, and Group 2-12 were administrated LPS by intranasal injection to induce experimental lung inflammation. After 1 day of LPS administration, Group 3-9 were treated with SCT × 1/4, × 1/2, × 1, × 3, × 6, × 12 or × 18, respectively. Group 10-12 (positive control) were treated with dexamethasone 1 ㎎/㎏ or acetylcystein 1.5 ㎎/㎏ or diclofenac·sodium 0.4 ㎎/㎏, respectively. After sacrifice, bronchoalveolar lavage fluid (BALF) was isolated. The levels of IL-1β, TNF-α, mucin glycoprotein 5AC (MUG5AC) were measured in BALF using enzyme-linked immunosorbent assay (ELISA). Results : LPS injected rats exhibited outstanding lung inflammation manifestations, including increased amount of total cells and neutrophil, and upregulated inflammatory cytokines level in BALF. However, the administration of SCT × 1/4, × 1/2 and × 1 decreased total cells and neutrophil, and suppressed the production of inflammatory cytokines, including IL-1β and TNF-α, and MUG5AC in BALF. Notably, inhibitory effect of SCT × 1/2 and × 1 on the level of TNF-α was markedly better than that of positive controls, dexamethasone and acetylcystein. Conclusions : Taken together, these results suggest that SCT × 1/2 and × 1 has therapeutic effects on LPS-induced lung inflammation rats model.